BIO-PRO
Bacteria Cultures
For
Waste Treatment Lagoons, Clarifiers, Digesters,
and Flow Through Ponds

BIO-PRO is a highly concentrated, proprietary blend of selected natural micro-organisms, essential nutrients, and synergists which help degrade the high concentrations of fat, grease, blood, floating matter, and organic matter found in processing wastes from meat plants, (poultry, fish, beef, pork, etc.). It is effective in anaerobic and facultative lagoons, aerated systems, and trickling filters. It degrades materials, which reduce BOD and COD, improves settle-ability in clarifiers, and reduces suspended solids. BIO-PRO will quickly destroy grease and fat buildup in grease traps, drains, sewer lines and lift stations, and is approved for use under the Federal Meat, Poultry, Shell Grading, and Egg Products Inspection Program. Regular use will also help:

Reduce overall sludge volume in clarifiers and treatment lagoons through enhanced biodegradation and help cause good settling characteristics.

Provide optimized degradation of organic wastes, including fecal, blood and fats from animal slaughter houses, meat and poultry produce processors.

Reduce the population of hydrogen sulfide forming bacteria and odors associated with them.

Improve anaerobic digestion and increase production of useful methane gas from indigenous methogenic bacteria, by providing partially digested substrate for these bacteria to further metabolize.

HOW IT WORKS:

When rehydrated and placed into an aqueous system containing certain essential nutrients, BIO-PRO cultures become the dominant organisms in the system and bio-convert the organic contaminants into fractions of smaller molecular weight. When maintained as the dominant organism in the treatment system through control of environmental conditions and periodic addition of fresh cultures, BOD/COD reductions of over 80% to 95% can be anticipated for many organic wastes within a few weeks.

BIO-PRO cultures are facultative anaerobes and achieve the bioconversion of organic compounds through both catabolic and metabolic enzyme digestion, under both aerobic and anaerobic conditions. Although BIO-PRO cultures function well in both conditions, their efficiency is improved if oxygen is plentiful in the treatment environment. Usually, shallow circulating bodies of water contain adequate levels of DO (dissolved oxygen).

Confined treatment sites such as stagnant lakes, ponds, drainage ditches, and municipal or industrial waste treatment clarifiers, digesters and lagoons should be artificially aerated to optimize bio-treatment efficiency.

In addition to carbon and oxygen, the bacteria require certain trace elements for successful growth. These include nitrogen, phosphorus, calcium, magnesium, zinc, iron, sodium and sulfur. Usually most contaminated sites contain adequate natural levels of these elements, however, it may be necessary under some conditions to add some elements to the treatment environment. This is especially true of nitrogen and phosphorus. A BOD:N:P ratio of 100:15:5 is optimal. When necessary, fertilization of the waste stream with a nitrogen phosphorus mixture will improve biodegradation efficiency. BIO-PRO can be used without fear of a malodor or ammonia problem.

ENVIRONMENTAL SAFETY:
BIO-PRO cultures are a blend of facultative anaerobes originally derived from the soil, which utilize non-living organic matter as a food source. The cultures are not harmful to either aquatic or land plants and animals and have been released into marine and fresh water treatment areas with ecological safety.

PACKAGING:
BIO-PRO is a free flowing white to tan colored powder and granules packaged in 25 lb. plastic pails and/or 100 lb. fiber drums. Each contain a plastic liner to reduce moisture absorption and sealing if the total container is not used at one time.

STABILITY:
When stored under normal conditions of temperature and humidity, the cultures are stable for a minimum of 3 years. Freezing has no significant effect on the cultures, however, they must be thawed prior to use. Excessive moisture may cause premature reactivation of cultures and a loss of potency.

PHYSICAL PROPERTIES:
Formulation: Dried mixed bacterial sapmrophytes of natural origin, combined in a mixture of dextrose, wheat grain, wetting agents and buffers.

PH of Hydrated Cultures: 6.8 to 7.2. Will not corrode equipment.

Potency > 3.0 x 107 organisms per gm. of powder, determined by total plate count.

HOW TO USE:

IN WASTE TREATMENT LAGOONS, CLARIFIERS, DIGESTERS,
AND FLOW THROUGH PONDS

TOTAL VOLUME

Total gallons or gallons per day

                    5,000          10,000
                     to              to             Over
                   10,000          300,000        300,000
                 ==========      ==========     ==========
Each day for
first 7 days      2 lb.per        1 lb.per       1 lb. per
                  5,000 gal.     10,000 gal.    10,000 gal.

Each day for
next 14 days      1 lb. per       1 lb. per      1 lb. per
                 10,000 gal.     20,000 gal.    40,000 gal.

Biweekly
thereafter        1 lb. per       1 lb. per      1 lb. per
                 10,000 gal.     20,000 gal.    50,000 gal.

Note: On site operator can best determine application rate to best suit varied conditions.

The effective use of BIO-PRO cultures in a bio-treatment program is dependent upon the environmental conditions present at the site. Adverse conditions such as cold temperatures, oxygen, nitrogen, or phosphorus deficient water, chemical toxic load, highly acidic/alkaline Ph or excessive dilution of biomass through inadequate dosage, may retard or prevent the cultures from becoming dominant in the treatment system. Under such conditions, a pilot scale treatibility study should be made to determine the organic degradation efficiency and dosage schedule.

BIO-PRO cultures are fully activated after soaking in water for up to 4 hours. To prepare, soak, using 2 gallons of warn tap or waste water for each 1 pound of BIO-PRO cultures. If the waste stream water is cool, the slurry should be cooled to the stream temperature just before application to avoid thermal shock. In lagoons or other treatment systems where the cultures will have long retention times, pre-soaking is not necessary, but the cultures should be added as a slurry.

BULK DENSITY: 6.7 lbs./gal. or 0.8g/cc.

GROWTH PARAMETERS:
The following physical and chemical parameters have been shown to be acceptable for growth of BIO-PRO cultures.

Ph levels: 5.5 to 8.5 is acceptable. Optimum will occur near neutral (6.6 to 7.4).

Temperature: 55'F to 100'F is acceptable. Optimum will occur 70'F to 90'F. Below 45'F there will be little to no growth, but cultures are not destroyed.

Nitrogen: Acceptable growth requires at least 5ppm. Doubling times will be most efficient at a nitrogen level of 20 ppm.

Phosphorus: Minimum level is 1 ppm with optimum growth at 7 ppm.

Toxic shock: BIO-PRO cultures are resistant to many toxic chemical shocks. Whenever chemical that have bactericidal activity are present, Pre-treatment studies should be run.

Guaranteed salmonella free.

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